| N-formylation of amines catalyzed by nanogold under aerobic oxidation conditions with MeOH or formalin | |
| Preedasuriyachai P., Kitahara H., Chavasiri W., Sakurai H. | |
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| Abstract | |
| Gold nanoclusters stabilized by poly(N-vinyl-2-pyrrolidone) (Au:PVP) are active and selective catalysts for N-formylation of amines under aerobic oxidation using methanol or formalin as a formyl source. © 2010 The Chemical Society of Japan. | |
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| Detection of the shrimp pathogenic bacteria, Vibrio harveyi, by a quartz crystal microbalance-specific antibody based sensor | |
| Buchatip S., Ananthanawat C., Sithigorngul P., Sangvanich P., Rengpipat S., Hoven V.P. | |
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| An immunosensor based on a quartz crystal microbalance (QCM) was developed for detection of Vibrio harveyi (V. harveyi), a pathogenic bacteria causing morbidity and even a high level of fatality in commercial shrimp cultures, including Penaeus monodon. Immobilization of a monoclonal antibody (MAb) against V. harveyi onto the gold electrode of a QCM plate involved a three-step procedure: (1) the formation of a self-assembled monolayer (SAM) of a carboxyl-terminated alkanethiol, (2) activation of the carboxyl groups by N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and (3) attachment of the MAb to the activated carboxyl groups. The developed QCM-based immunosensor could be used for the detection of V. harveyi in a working range of 103-107 CFU/mL, with no detectable cross-reactivity to Vibrio vulnificus and Vibrio parahaemolyticus. Controlling the density of surface-immobilized MAb, by the formation of mixed SAMs containing hydroxyl-terminated alkanethiol in combination with the treatment of 1% (w/v) bovine serum albumin (BSA) as a blocking reagent, can significantly improve the binding efficiency of the targeted bacteria. © 2009 Elsevier B.V. All rights reserved. | |
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| Alkanethiols; Binding efficiency; Bovine serum albumins; Carbodiimides; Carboxyl groups; Cross-reactivity; Gold electrodes; Hydroxyl-terminated; Mixed SAM; Monoclonal antibodies (mAb); N-hydroxysuccinimide; Pathogenic bacterium; Penaeus monodon; Shrimp culture; Vibrio parahaemolyticus; Vibrio vulnificus; Working range; Bacteriology; Body fluids; Chemical detection; Immunosensors; Monoclonal antibodies; Oxide minerals; Piezoelectric devices; Quartz; Quartz crystal microbalances; Self assembled monolayers; Surface plasmon resonance; Bacteria | |
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